Nassau Tower was the Long Island Rail Road's interlocking and signal tower for NASSAU Interlocking at Mineola Junction, just east of the Mineola station, from 1923 until 2020.

[4] In the 2010s, it was announced by Governor Andrew M. Cuomo that the main Line would finally receive a third track, after decades of discussions, debates, and proposals.

[1][5][6][7] The construction of this expansion would require either demolishing or moving the tower, as the space would be needed for the new track, with the MTA ultimately preferring the former option.

[5] As a result, many organizations, including the Mineola Historic Society, the Oyster Bay Railroad Museum, as well as many railroad enthusiasts and locals, tried to preserve the building by having it moved and transformed into the Mineola Railroad Museum, citing an MTA proposal from 2005, the history of the building, and its eligibility for receiving both state and national historic statuses, as well as being considered a local landmark by many in the Mineola community.

[1] In March 2021, while contractors were demolishing an adjacent electrical substation that had been deactivated, debris fell onto Nassau Tower.