National Route 25 is a 534-kilometer-long paved highway that runs through the center of Chubut Province, Argentina.

It extends from the juncture with National Route 40 in Tecka to the bridge on the Chubut River in the city of Rawson.

This route was established by the Tehuelche people to journey between the coasts in the winter and the Andes in the summer.

After the establishment of Welsh colonies in the valley below, and encouraged by news sent from the Andes by Wisel (also spelled Huisel, Wissale or Wishel) and then by other caciques, John Daniel Evans ventured on this route in late 1885 together with Governor Luis Jorge Fontana in an expedition called Riflemen of Chubut (Rifleros del Chubut).

Years later and after a second expedition, Evans guided the first group of colonists to definitively establish themselves in the mountain range, linking the coast and the October 16 Colony (Colonia 16 de Octubre) (renamed Trevelin in 1918) on November 20, 1888.