The Nativity of the Virgin is a painting by the Italian late medieval painter Pietro Lorenzetti, dating from around 1335–1342, now housed in the Museo dell'Opera del Duomo of Siena, Italy.

All the paintings should represent stories of the Life of the Madonna, and were crowned by Duccio di Buoninsegna's Maestà.

The left scene depicts Joachim, Mary's father, waiting together with a child and an old man.

St. Anna is lying on a bed (a typical medieval chest) in the central scene with a white drape behind, while, in the foreground, two women are washing her daughter.

A woman, wearing a red dress, is shown behind the right pier, while speaking with Anna; she holds an inlaid object, perhaps a banner or a musical instrument.