The first class was composed of 25 young women who began their studies in a large mansion on Lake Avenue in Rochester, New York.

[6] The campus has 24 buildings, including 11 residence halls, a 2,200-seat stadium, and all-weather track, located on 150 acres (0.61 km2).

It is the oldest and largest building on campus, once serving as the "motherhouse" for the Sisters of St. Joseph who founded Nazareth.

[citation needed] The Golisano Training Center (opened in fall 2019), is a multi-use facility supporting varsity athletics, Special Olympics events, fitness, and a wide range of campus activities.

Athletic facilities at Nazareth include the Robert A. Kidera Gymnasium (1,200), Golden Flyer Stadium (2,200), and the Golisano Training Center, a full size indoor field house with an indoor track, tennis courts, and turf field.

[citation needed] The Nazareth men's lacrosse team is a three-time NCAA Division III National Champion (1992, 1996, and 1997).