It is the largest depot on the London Underground, and is currently responsible for maintenance and overhaul of the 191 S Stock trains used on the Circle, District, Hammersmith & City and Metropolitan lines.

Major work was undertaken at Neasden between 2010 and 2011, with much of the 1930s layout being altered to make it suitable for maintenance of the S Stock, which began to be rolled out in 2010 to all London Underground Sub Surface lines.

[3] Due to the rebuild, Neasden's steam shed has now been decommissioned and converted into the Depot's training facility.

[6] Neasden is now the main depot for the Metropolitan line, stabling passenger trains, and works vehicles including battery electric locomotives and wagons.

Access to the depot by road is just off Neasden Lane, and is the home to one of only a handful of level crossings on the Underground.