Nebulin has also been shown to regulate actin-myosin interactions by inhibiting ATPase activity in a calcium-calmodulin sensitive manner.

[8] A smaller member of the nebulin protein family, termed nebulette, is expressed in cardiac muscle.

Bang and colleagues[10] demonstrated that nebulin-knockout mice die postnatally, have reduced thin filament length, and impaired contractile function.

Postnatal sarcomere disorganization and degeneration occurred rapidly in these mice, indicating the nebulin is essential for maintaining the structural integrity of myofibrils.

Witt and colleagues[11] had similar results in their mice, which also died postnatally with reduced thin filament length and contractile function.