Pendse started her acting journey as a child artist in Ekta Kapoor's horror show Captain House back in 1995, when she was just ten years old.

Her film debut happened in 1999 with an uncredited appearance in Daag: The Fire, followed by playing Sunny Deol's sibling in Pyaar Koi Khel Nahin.

[3] Pendse began her acting journey at the tender age of ten, making her debut as a child actor in the television series Captain House in 1995, under the production of Ekta Kapoor.

[5] Venturing into episodic television, she showcased her versatility through various roles in Rishtey–The Love Stories, delving into the complexities of human relationships with nuance and depth.

In 1999, she marked her Hindi film debut, although uncredited, in Daag: The Fire, and the same year, she portrayed Sunny Deol's sister in Pyaar Koi Khel Nahin.

[6] Throughout this period, she often portrayed the roles of siblings to the main protagonists in Hindi cinema, featuring in films like Deewane (2000), Chhupa Rustam: A Musical Thriller (2001), Tum Se Achcha Kaun Hai (2002).

[8][9] Additionally, during the same year, she made her debut in Tamil cinema with the romantic drama Mounam Pesiyadhe, starring alongside Suriya, Trisha and Nandha.

[10] The following year, Pendse appeared alongside J. D. Chakravarthy in the Telugu comedy Golmaal, which was a remake of the 1994 Malayalam film Malappuram Haji Mahanaya Joji.

Malathi Rangarajan of The Hindu praised her portrayal, stating, "Neha, whom we saw as the second heroine in Mounam Pesiyathe is apt as the bold, belligerent Mamathi later softened by love.

[citation needed] That same year, she got her first lead role in a Hindi movie titled Dreams, but it didn't do well with critics or at the box office.

[citation needed] Portraying the lead character of a troubled woman named Kashi, she starred alongside Vikram Gokhale, Priya Berde, and Surekha Kudachi.

She played a supporting role in Rajendra Talak's A Rainy Day and took on the female lead in the fictional biopic Dusari Goshta, directed by Chandrakant Kulkarni, based on the life of former Home Minister Sushilkumar Shinde.

"[35] In 2015, she starred opposite Umesh Kamat in Balkadu, a film inspired by the ideologies of the late Indian politician Bal Thackeray.

[37][38] Keertikumar Kadam of Marathi Movie World praised her performance, stating that "Pendse does what was expected of her and looks ravishingly stunning, on screen.

"[39] Following this, she appeared in Anant Mahadevan's biographical film Gour Hari Dastaan, which depicts the life of an Indian freedom fighter from Odisha.

[45] This cinematic adaptation, based on a play by the renowned playwright Kusumagraj, delves into the poignant family dynamics of a retired stage actor grappling with nostalgia for his past life in the theater.

[47][48] The following year, she had only one film release, Nagarsevak - Ek Nayak, which received negative reviews, starring opposite Upendra Limaye.

In 2021, Pendse took over the role of Anita Vibhuti Narayan Mishra in the popular daily sitcom Bhabiji Ghar Par Hain!, replacing Saumya Tandon.

Her next project was the Marathi drama film June, set in Aurangabad, tackling various social issues such as bullying, sexism, suicide, and the generation gap.

[58] Critics applauded Pendse's performance, with Mihir Bhanage from The Times of India describing it as a "revelation" and noted, "she channels Neha's inner turmoil effortlessly and is comfortable in the skin of the character.