[1] In 1940, the Alfred I. duPont Institute, a pediatric orthopedic hospital, opened on the grounds of the Nemours Estate in Wilmington, Delaware.

After his death in 1981, a substantial portion of his wealth was bequeathed to the foundation, significantly contributing to the expansion of Nemours' services, particularly in Florida.

[6][7] The hospital expanded throughout Florida in the 1990s, opening Nemours Children's Clinic locations in Jacksonville, Orlando, and Pensacola.

[1] Nemours Children's Health opened a specialty care and ambulatory surgical center in Malvern, Pennsylvania in March 2024.

[9] Nemours operates major research centers and laboratories, fostering partnerships with academic institutions and companies.