Neoholothele is a genus of tarantula, first described in 2015 by Guadanucci & Weinmann.

As of August 2022[update], it contains 2 species Neoholothele fasciaaurinigra and Neoholothele incei, the latter being the type species.

[1] They are named after the prefix "neo" from the Greek word for new, and the genus Holothele.

[2] They can be distinguished by the coloration of the carapace, which is dark with a golden cephalic region.

Females with a striped pattern on the abdomen, with a long and slender spermathecae receptors.