It was serialized in Shogakukan's Weekly Post [ja] from November 2003 to December 2006, with its chapters collected in 11 tankōbon volumes.

The two soon become enmeshed in a plot by the Shogunate conceived by the ruthless Matsudaira Nobutsuna and spearheaded by his chief henchman Mamiya Rinzō (also based on an actual historical character) to topple the Satsuma clan and assume control of that fiefdom's great wealth, using Tōgō as an unwitting pawn.

When Tōgō discovers that he has been tricked and used, he and Daigoro embark on the road of meifumado in a quest to kill the Shogun (which would force Matsudaira out into the open).

Written by Kazuo Koike and illustrated by Hideki Mori, New Lone Wolf and Cub was serialized in Shogakukan's Weekly Post [ja] from November 10, 2003,[3] to December 18, 2006.

[11][2] The series resumed on eBookJapan [ja]'s online manga magazine Katana on April 14, 2009,[12][13] and finished on July 20, 2010.