It is notable for its resident glow-worms, the bioluminescent larvae of Arachnocampa richardsae, a type of fungus gnat.
The tunnel is now contained within the Wollemi National Park and is a popular attraction for bushwalkers and tourists.
Outside the tunnel, the area features spectacular gorges, caves and scenery.
On the south side of the tunnel, a large gap in the road prevents vehicular access.
According to some sources, this gap was created deliberately to keep cars out of the tunnel, because the exhaust fumes would have killed the glow-worms.