Between the early 1560s and the mid to late 1570s, Mosley returned to the Manchester area (where five of his children were baptized at St James' Chapel, Didsbury, between 1562 and 1568).
In 1592 (following the death of his first wife) he married the widow Elizabeth Hendley (née Rookes) at All Hallows, Bread Street in the City of London.
His mayoralty was notable for the fact that there was a significant threat of Spanish invasion during that year, and Mosley was required to raise funds and forces for the defense of London.
"[2] The text further states that, "It became a fashionable practice in the reign of Queen Elizabeth [I] to assume some motto bearing a quaint allusion to the family name, and in conformity with [this fashion], as well as out of compliment to his youngest son, Edward, who was just then rising to eminence in his profession as a barrister, Sir Nicholas adopted the Latin words, 'Mos legem regit' ('Custom, or precedent, rules the law')."
Mosley died in 1612 and was buried in the Church of St James, Didsbury, where a monument shows him kneeling, "dressed in the robes of the Lord Mayor of London".