Shorts selected for the festival had the chance to be aired on Nicktoons Network, online and to be showcased at a live event in Los Angeles in October.

[4] Frederator Studios has persisted in the tradition of surfacing new talent, characters, and series in several ways, with The Nicktoons Film Festival being the first to present short cartoons recently produced by independent filmmakers around the world.

Some of the most notable entries include To a Man with a Big Nose, Bathtime in Clerkenwell, Kiwi!, Kenya and Magical Trevor, the Ape Escape trilogy of shorts, The Naive from Loli Land, Charlie & Chunk, The Mouschist, Prey, Hero: 108, Bert, Engrish Bwudd, Skippy, Monster on the Moon, Pigly, Libraryhead, Over the Moon, Feb-18-05, Timmy's Lessons In Nature, Farm Force, Late Night Comedy, Zoologic, Maestro, Zoya the Zebra, PGi-13, The Tale of How, Loco Melones, Rockfish, Super Scout and Welcome to My Life.

and both weebl's Kenya and Magical Trevor were considered major internet memes at that time, The Mouschist was created by Courage the Cowardly Dog creator John R. Dilworth, the Ape Escape trilogy of shorts promoted the game series exclusively for Sony's own PlayStation 2 at the time (and had eventually aired its own series on Nicktoons five years later), and the four were precursor shorts—Super Scout, The Naive from Loli Land, Hero: 108 and Welcome to My Life—would eventually become precursor to Nickelodeon's own The Mighty B!

[6] These laboratories have spun off notable series like: Dexter's Laboratory, The Powerpuff Girls, Johnny Bravo, Cow & Chicken, Courage the Cowardly Dog, Larry & Steve (which was the basis for Fox's own Family Guy), Kenny and the Chimp (which was the basis for Codename: Kids Next Door), Grim & Evil (which was split into Evil Con Carne and The Grim Adventures of Billy & Mandy), The Fairly OddParents, My Life as a Teenage Robot, ChalkZone, Nite Fite, Fanboy & Chum Chum, Adventure Time, Bravest Warriors, Rocket Dog, and Bee and PuppyCat.