Created by David Kaler and Steve Ditko, the character first appeared in Captain Atom #82 (September 1966) originally published by Charlton Comics.

Her mother, Magda, was actually a visitor from another dimension whose denizens have the ability to transform into living two-dimensional shadows; she passed these powers on to her son and daughter.

Once integrated into the DC Universe in Crisis on Infinite Earths, Nightshade was revamped as a government super-spy who worked for Task Force X aka the Suicide Squad.

Taking the name Chimera, Nightshade was forced to participate in the massacre of innocent civilians at an airport as Jihad sought to demonstrate their effectiveness to a potential client.

As a result of the mission with Jihad, Nightshade was removed from her position as a spy and given the job of being the handler for the mentally unstable sorceress known as the Enchantress.

Eve Eden's mother Maureen was the queen of the Land of Nightshades, and fled to Earth with her infant children to escape the demonic power of an entity known as the Incubus.

Eve believed she was a normal child until her mother took her and her brother Larry into the Land of Nightshades, to show them their true heritage.

The Incubus possessed Larry's body, killing him in the process, and hid within the Land of Nightshades slaughtering all remaining human inhabitants.

The two entities sought to possess Larry and Eve to force the siblings to conceive a child who would be the incarnation of their demonic master.

With the Succubus defeated, Nightshade passively watched as her teammate Deadshot finished the mission, killing the Incubus by shooting the entity in the head.

During the Superman/Batman story arc "Public Enemies", she was for a brief time under the control of Gorilla Grodd, trying to capture Superman for a billion-dollar reward.

During the Infinite Crisis crossover she joined a legion of DC's magic-based characters battling the Seven Deadly Sins, but was captured by Felix Faust and eventually used by Alex Luthor to bring back Earth-4.

In Shadowpact #7 it was revealed that she requires some measure of concentration to form elaborate darkness constructs, when she and her partner Ragman were attacked by the Congregation.

Her powers restored, Nightshade joins the combined Suicide Squad and Secret Six members in fighting off the Black Lanterns.

Nightshade sends a living shadow to attack Deathstroke and grab the box with the weapon on a memory key after he had extracted it from an ice wall.

Deathstroke assumes that Amanda Waller has something to do with this, but Nightshade responds that both he and she herself aren't with the Suicide Squad anymore but are still tangled with US intelligence operations.

She can't transport Deathstroke to the Land of Nightshades, because the promethium in his equipment is capable of absorbing most types of energies including the Night Force.

Instead, she uses her shadow-matter raven Hugin to transport Robin (Damian Wayne), who Deathstroke is working with begrudgingly, to the Land of Nightshades (where humans are driven insane as they're faced with their worst nightmares) to force Deathstroke to open the box and give the weapon inside to her, because she can't safely open it herself without setting off the explosives in the box.

[14] In Doomsday Clock, she appears alongside Blue Beetle, the Question, and Captain Atom in the Bug airship as they travel to Mars.