516 Squadron RAF was an army co-operation squadron of the Royal Air Force during the Second World War.

It operated a large variety of aircraft with the unusual task to provide realistic low-level attacks against commando and assault troops undergoing training.

The work involved detaching aircraft near the troop training areas including RAF Ayr and RAF Haverfordwest.

By 1944 the training of commando and assault troops was on the decline and the squadron was disbanded at Dundonald on 2 December 1944.

No squadron codes are known to have been carried between April 1943 and Dec 1944.