The route travels between Whitsett and Burlington, connecting the cities of Gibsonville and Elon.

At the turn south onto Maple Avenue, NC 100 goes through a mostly residential area for 1.1 miles (1.8 km), before ending at the intersection with NC 54/NC 49 (Chapel Hill Road/Harden Street).

[1] At Burlington city limit, the North Carolina Railroad/Norfolk Southern tracks cross over the highway along a polygonal Warren truss, built in 1935.

[2] The first NC 100 was established in 1929 as a new primary routing from US 70/NC 10 in Whitsett to US 70/NC 10/NC 62, in Haw River.

[8] In 2014, an elimination of an at-grade intersection at Burlington Avenue and University Drive was made; NC 100 was rerouted on a new connector road from Burlington Avenue/Haggard Avenue to University Drive.