Its western terminus is at U.S. Route 62 (US 62) about four and a half miles (7.2 km) south of Russellville, and its eastern terminus is near the unincorporated community of Decatur at SR 125.

The entire route is a two-lane highway, passing through farmland and woodland.

SR 353 begins at an intersection with US 62, immediately north of US 68, in the unincorporated community of Redoak.

The highway makes a sharp turn towards the east, leaving the creek, and passing through woodland.

[4][5] This state highway runs entirely within the eastern part of Brown County.