It was built down the side of a wooded ravine formed by one branch of the Don River located in Flemingdon Park.

[5] The same day, the Ford government announced that the site would be closed immediately and permanently, without attempting repairs due to the pending relocation.

The Communications room contained a number of computerized displays, including a very popular tic-tac-toe game, run on a PDP-11 minicomputer.

[14] The Ontario Science Centre later amended the contract to specify that all goods sold to Oman would be produced in North America.

The Agents of Change transformation was completed 2007, culminating with the opening of the Weston Family Innovation Centre and the Teluscape plaza.

[17] In response to the COVID-19 pandemic in Ontario, the Science Centre received $500,000 from the Government of Canada to promote COVID-19 vaccine uptake among children and their families.

[18] Due to structural decay, which required closing of the pedestrian bridge leading to the exhibits, a shuttle bus line ran from 2023 until the Don Mills site's closure, bringing people from the main entrance to Level 6, the main exhibit area at the bottom of a ravine.

On April 18, 2023, Doug Ford, the premier of Ontario, announced the provincial government's plan to replace the existing Science Centre with a new facility on the Toronto waterfront.

[2][3][22] The proposed new location would be substantially smaller than the current one, requiring the Science Centre to downsize[21] and potentially remove certain exhibits.

[24] Toronto City Council strongly opposes the Ontario provincial demolition plans, and is investigating alternative ways to continue museum operations in the current location.

[23] The Architectural Conservancy of Ontario (ACO) and Toronto Society of Architects (TSA) have issued separate statements opposing the demolition of the original buildings.

The report included a recommendation to restrict access to occupied spaces below the compromised roof panels, followed by reinforcement or replacement by October 31.

[31] Instead of pursuing repairs, the Ford government opted to expedite its plan to relocate the Science Centre to Ontario Place, targeting a 2028 opening.

[6][32] The decision to close the Don Mills location drew criticism from various groups, including Moriyama Teshima Architects (the architectural firm founded by the Science Centre's designer).

[5] At least three donors with connections to science and technology, including Geoffrey Hinton, have made donation offers of up to $1 million to cover the costs of the emergency repairs, while Moriyama Teshima Architects offered to provide consulting services related to the repairs at no charge.

[39] The Great Hall is an event venue at the Ontario Science Centre and is home to Cloud, a massive, computer-controlled kinetic sculpture by Toronto installation artist David Rokeby, which consists of an array of blue and transparent squares that rotate in various ways to simulate the three states of matter: solid, liquid and gas.

Lotic Meander by Stacy Levy is an outdoor installation in polished and blasted granite and cast glass set into the solar terrace of the Ontario Science Centre.

As originally built, the Science Centre had a large fountain area directly in front of the entranceway, located to create a traffic roundabout.

The exhibition features eight areas that comprise different interactive components, historical artifacts, informational walls, documentary videos and more.

In addition to detailing anatomy, the exhibit explores the possibilities of the human body with activities to simulate the experiences of adventure-seekers, elite athletes, and extreme-sports enthusiasts.

There are more than 80 exhibits in the hall, which were all developed and built by the Science Centre's staff with input from more than 120 neuroscience, physiology, bio-mechanics and sports medicine experts.

[55] The Cohon Family Nature Escape is an outdoor exhibit, situated within the Don River valley to the rear of the science centre.

The exhibit features a giant Baltimore oriole nest, concrete wall canvas for moss graffiti, and a playground slide made from a fallen 125-year-old eastern white pine.

The exhibit includes a 464-year-old Douglas fir, with markings on its growth rings denoting notable world events during the tree's lifetime.

[55] The Space Hall was refurbished in the late 2000s and features meteorites from Mars and one of the few Moon rocks on public display in Canada.

[55] The Weston Family Innovation Centre houses Pipe Dreams by Bruce Shapiro, a bubble art installation.

[63] In his CBC news satire program, Rick Mercer visited numerous scientists' exhibits, took part in a wildlife conservation show, and partook in a psychological fear study during a workshop there.