Oosteeklo (formerly Oost-Eekloo) is a historic village in the Flemish province of East Flanders, Belgium, which since 1977 has been a subdivision of the municipality of Assenede.

From 1217 until 1577 Oosteeklo Abbey, a Cistercian nunnery, was located in the village, but the community relocated to Ghent for safety during the hostilities of the Dutch Revolt.

[2] The monastery did maintain some property rights in the village, and a guesthouse once owned by the nuns is now listed built heritage.

The soil was sandy and the landscape low-lying heathland, granted to Oosteeklo Abbey and sublet to tenant farmers.

On 18 October 1790 a corps of volunteers from Oosteeklo was sworn into the army of the United Belgian States in Ghent.