[1] Orsay virus has a (+)ssRNA genome consisting of two segments, termed RNA1 and RNA2.

The RNA1 segment encodes an RNA-dependent RNA polymerase (RdRP) of 982 amino acids.

Just before the time of the discovery, standardized methods were developed for the wild isolation of nematodes.

Electron microscopy showing viral particles confirmed the role of a virus.

[3] On infection of a susceptible nematode, the viral particles are mainly localized to intestinal cells.