It has won India Biodiversity Award 2016 in the category of 'Conservation of threatened species' for its Hornbill Nest Adoption Programme.

The terrain is rugged with mountainous ranges in the north and narrow plains and sloping hill valleys in the south.

The forest has a typical layered structure and the major emergent species are bhelu Tetrameles nudiflora, borpat Ailanthus grandis and jutuli Altingia excelsa.

The general vegetation type of the entire tract is classified as Assam Valley tropical semi-evergreen forest.

The forest types include tropical semi-evergreen forests along the lower plains and foothills dominated by kari Polyalthia simiarum, hatipehala Pterospermum acerifolium, karibadam Sterculia alata, paroli Stereospermum chelonoides, Ailanthus grandis and khokun Duabanga grandiflora.

[6] The tropical semi-evergreen forests are scattered along the lower plains and foothills, dominated by Altingia excelsa, nahar Mesua ferrea, banderdima Dysoxylum binectariferum, Beilschmedia sp.

Along the larger perennial streams, there are shingle beds with patches of tall grassland, which give way to lowland moist forests with outenga Dillenia indica and boromthuri Talauma hodgsonii.

Along the larger rivers, isolated trees of semal Bombax ceiba and two species of koroi Albizzia sp.

[2] At least 296 bird species have been recorded from PTR including the globally endangered white-winged wood duck, the unique ibisbill, and the rare Oriental bay owl.

[8] Birds seen in Pakke Tiger Reserve include: Jerdon's baza, pied falconet, white-cheeked hill-partridge, grey peacock-pheasant, elwe's crake, ibisbill, Asian emerald cuckoo, red-headed trogon, green pigeon spp., forest eagle owl, wreathed hornbill, great hornbill, collared broadbill and long-tailed broadbill, blue-naped pitta, lesser shortwing, Himalayan shortwing, Daurian redstart, Leschenault's forktail, lesser necklaced laughing-thrush, silver-eared leiothrix, white-bellied yuhina, yellow-bellied flycatcher warbler, sultan tit, ruby-cheeked sunbird, maroon oriole, and crow-billed drongo.

Presently, there are 27 anti-poaching camps where 104 local youth and 20 gaon buras (village fathers) have been employed as forest watchers.

[11] The Nyishi community has joined hands with civil society and the forest department to protect hornbill nests.

The Nyishi tribe uses fiber glass replicas of hornbills beaks as their headgear and has fines for hunting of tigers, among other regulations.

A group of 12 village heads, along with the forest department, supports conservation efforts around Pakhui Tiger Reserve (PTR).

[12] The Ghora Aabhe enforce customary laws, institute penalties against hunting and logging, aid in capacity building and spread awareness of PTR.