The Palazzo al Canto di Sant'Anna is a building in Florence, composed of two bodies, with one façade on Via de' Pepi 67 and one on Via Fiesolana 32.

This is a large palazzo that on Via Fiesolana shows seven axes on three floors, with the central doorway surmounted by a balcony, the result of a late 19th century renovation carried out on a block of pre-existing houses.

[1] The building is mentioned by Gabriella Orefice as an example, in the Santa Croce district, of "...a successful operation of transformation of the ancient, dilapidated blocks of flats into a dignified building for bourgeois use, with innovative features compared to the recurring Florentine typologies, particularly if one considers the narrow and deep courtyard, in axis with the entrance portal, along which long balconies supported by corbels develop".

In reality, the paintings are found in the entrance hall on the Via Fiesolana side and are related to a late 16th century taste, with scenes of the Genesis between grotesques.

Such subjects would suggest the presence in antiquity of a religious institute, perhaps dedicated to St. Anne, whose tabernacle existed not far away, giving its name to the nearby Canto di Sant'Anna.