The female mosquitoes were originally collected in 2010 from Darwin, Katherine, Alice Springs, Alyangula, Groote Eylandt, Jabiru and the McArthur River Mine, and had since been preserved.

[1] The follow-up experiment confirmed the fact that pre-infection of mosquitoes prevents transmission of harmful arbovirus.

Further, it shown that PCV could not infect Culex annulirostris, the primary vector of encephalitic flaviviruses in Australia, through blood meal indicating that the virus is not a threat to human, or other vertebrate, health.

However, the virus could be inoculated (at the thorax region) with successful infection in the C. annulirostris, Aedes aegypti and A. vigilax.

[4] Duplicated xrRNAs in PCV appear to be evolutionarily selected for to provide functional redundancy allowing the production of sfRNAs if one structures is disabled by mutations.