[1][3] The church seems to have obtained yet another intervention by the Knights, which is dated around the second half of the 15th century and is probably connected to the siege of 1480, during which Panagia tou Kastrou suffered extensive damage.

[2] The church of Panagia tou Kastrou is located at the eastern end of the Street of the Knights, in the Kollakio of the medieval city, near the seaport.

The original core of the building was a cruciform inscribed with dome type; this architectural style is evident until approximately the genesis of arches.

[1][3] After the capture of Rhodes by the Knights Hospitaller, the Byzantine church was remodeled and took the form of a three-aisled Gothic basilica with a transversal aisle.

On the west side, above the central entrance door, a large rectangular frame is preserved, which would have housed a painted composition, that is now lost, with the subject of Virgin Mary surrounded by saints and knights.