[3] As a young man, Mascagni was interested in geological sciences, as evidenced by his several papers on the Lagoni (thermal springs) of Siena and Volterra.

His many discoveries in this field led to the composition and publication of Vasorum lymphaticorum corporis humani historia et iconographia in 1787.

[2] He was elected a corresponding member of the Royal Swedish Academy of Sciences in 1796, and president of the Accademia dei Fisiocritici in 1798.

Mascagni was freed from prison by a motu proprio of the King of Etruria, who on 22 October 1801 appointed Mascagni a professor of anatomy at the University of Pisa, with the additional charge of lecturing twice a week at the Hospital of Santa Maria Nuova in Florence.

[3] In 1781, Mascagni began advising sculptor Clemente Susini, who was working on a collection of human anatomical waxes.