Chiusdino is a comune (municipality) in the Province of Siena in the Italian region Tuscany, located about 70 kilometres (43 mi) south of Florence and about 30 kilometres (19 mi) southwest of Siena.

Chiusdino borders the following municipalities: Casole d'Elsa, Monticiano, Montieri, Radicondoli, Roccastrada, Sovicille.

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