[13] Gemperline came to the notice of a larger scientific community in 1984 with the publication of a paper describing DISNET in the Journal of Automated Methods and Management in Chemistry.
Gemperline and his colleagues provided methodologies which underlay the improvements in calibration accuracy, computer-based data acquisition and mathematical analysis in chemometrics.
[18] Gemperline collaborated with Burroughs Wellcome in the 1980s to develop software for multivariate pattern recognition analysis of near-infrared reflectance spectra for rapid, non-destructive testing of pharmaceutical ingredients and products.
[17] Cutler notes that Gemperline, "built his career and has produced world-renowned chemometrics research with the limited resources of mostly undergraduates and master's level students.
[17] He collaborated with David Haaland at Sandia National Laboratories to develop chemometric tools to study kinetics in cells using hyperspectral fluorescence imaging.
Their efforts were judged successful, and led to the 2010 Meggers Award for them and coauthors Patrick Cutler and Erik Andres for their two 2009 publications in Applied Spectroscopy about their research.
[7] Outside of his scientific, teaching and administrative pursuits, Gemperline enjoys cycling, sailing, kayaking, photography[15] and watching Dancing with the Stars on TV.
The chapters he contributed were based on the teaching notes he had developed at East Carolina University for use with undergraduate and master's degree level students.