Pennichaw is a rural unincorporated community located in south Volusia County, Florida, United States.

Built from a connection with the Plant System near the steamboat landing in the village of Enterprise, southeasterly through Osteen and Maytown, eventually reaching the coastal town of Titusville in 1885.

Known as the Kissimmee Valley Branch and heading southwest from the busy railroad town of New Smyrna Beach, the new route passed through Maytown-PENNICHAW-Osceola-Geneva and was completed to Chuluota in 1912.

However, before it would be abandoned, the Peavy Wilson Lumber Company, operating a massive mill in Holopaw, leased the Holopaw-Pennichaw-Maytown trackage.

[4] The East Central Regional Rail Trail corridor, which is more than 50 miles long, is managed by Volusia and Brevard counties.