PA 741 forms a concurrency with U.S. Route 222 (US 222) between Willow Street and Lampeter before it continues east through farmland in the Pennsylvania Dutch Country that is home to several Amish families, passing through Strasburg before reaching Gap.

From this point, PA 741 heads south as a three-lane road with a center left-turn lane that is locally maintained, passing through industrial parks.

At this point, PA 741 becomes McGovernville Road and comes to a bridge over Norfolk Southern's Lititz Secondary and Amtrak's Keystone Corridor railroad lines.

PA 741 passes homes to the west and a branch of Lancaster General Hospital to the east prior to coming to the US 30 interchange.

Following US 30, the route becomes a two-lane undivided road and passes residences prior to crossing Norfolk Southern's Columbia Secondary railroad line at-grade and entering the residential and commercial community of Rohrerstown.

[2][3] After this intersection, PA 741 enters Manor Township and becomes Millersville Road, passing farm fields to the west and suburban residential neighborhoods to the east.

Farther south, the road enters areas of woods and homes, making a turn to the southeast and crossing Little Conestoga Creek.

At this point, PA 741 continues into Pequea Township and runs through agricultural areas prior to entering the residential community of New Danville.

[2][3] Upon reaching the community of Willow Street, PA 741 widens into a two-lane divided highway and meets the southbound direction of US 222/PA 272.

The two routes continue east on Beaver Valley Pike, passing farms to the north and commercial development to the south.

[2][3] At this point, the route enters the Pennsylvania Dutch Country of eastern Lancaster County, which is home to many Amish farms.

The route continue past more homes on East Main Street prior to crossing into Strasburg Township and meeting PA 896.

[7][8][9] In 1928, the road between Willow Street and Gap was designated as part of PA 41, which ran from Harrisburg southeast to the Delaware border.