The Museum was officially opened on 2 June 2001 by Jackie Kelly, MP for Lindsay, the then Minister for Sport and Tourism as well as with the support of industry organisations and a Commonwealth Government Federation Fund Grant.

In September 2017 the Penrith Museum of Printing closed its doors for a major upgrade and added 150 m2 to their premises including the addition of a foyer and a library.

The Penrith Museum of Printing houses a collection of fully operational letterpress machinery and equipment.

The museum featured in the 2018 film Ladies in Black, where it was used to simulate the Sydney Morning Herald's compositors' room.

[2] Used to produce local France newspaper Le Courrier Australien and the France-Australian trade magazine France-Australie The Penrith Museum of Printing has an extensive collection of books, manuals, documents and other letterpress printing artefacts which are all available for viewing.