The Perdido Key beach mouse is a nocturnal animal, spending most daylight hours in their burrows.

Unlike many species, beach mice are monogamous, with mated pairs tending to remain together as long as both live.

In contrast to its inland relatives, the Perdido Key beach mouse avoids humans, buildings and trash, instead preferring to meander among the dunes near its burrow.

Mouse burrows are usually located in the dunes at the base of a shrub, clump of grass or near some vegetated cover.

If an intruder, such as a snake or crab, enters a beach mouse burrow, mice make a hasty retreat out the escape tube.