Regional train services calling at the station are operated by Ferrovia Centrale Umbra, which sub-contracts on behalf of Trenitalia.

Perugia railway station is situated at Piazza Vittorio Veneto, in the heavily populated district of Fontivegge, about 3 kilometres (1.9 mi) southwest of the city centre.

The station was opened on 12 December 1866, upon the inauguration of the Ellera Corciano–Ponte San Giovanni section of the Foligno–Terontola railway.

Dividing the ticket hall from the station yard are three blue and green glass doors.

Passengers inside the building can also admire a sculpture from Roman times in honor of the sacrifice of Perugia in the war against Hannibal.

In the former goods yard there are some free tracks and an electrification dock, all of which serve today for the storage of disused or out of service carriages and line maintenance equipment.

Regional services are operated by Ferrovia Centrale Umbra, which sub-contracts on behalf of Trenitalia.

Their main destinations are Firenze Santa Maria Novella, Terontola-Cortona, Foligno and Roma Termini.