Picovirinae is a subfamily of viruses in the order Caudovirales, in the family Salasmaviridae.

[2][3] The following genera and species are recognized:[3] Viruses in Picovirinae are non-enveloped, with icosahedral or prolate heads of about 50–55 nm in diameter, and short tails.

Genomes are linear, double stranded DNA, and are relatively small (between 16–20 kbp)-hence the term pico-virinae.

Picoviruses package linear, monomeric genomes with a terminal protein covalently attached to each end.

Replication follows the DNA strand displacement model.