The Pittsburgh and Ohio Central Railroad (reporting mark POHC) is a short-line railroad operating 35 miles (56 km) of track over the Chartiers Branch in southwest Pennsylvania.

It also operated a small portion of the former Conrail Panhandle Route between Carnegie and Walkers Mill.

It is owned by the Ohio Central Railroad System, which is a division of the rail holding company Genesee & Wyoming Inc.

The P&OHC was formed after the purchase by Genesse & Wyoming of the former Pittsburgh Industrial Railroad from RailAmerica, Inc. in 2000.

Major commodities hauled include chemicals, minerals, plastics, and steel.