On and along the Plantage Muidergracht, near Roeterseiland, are some of the buildings of the University of Amsterdam, originally built for courses or subfaculties of physics, chemistry, biology, environmental science and mathematics, but now used by other faculties.

The canal runs in a southeastern direction from the Nieuwe Keizersgracht, at the Hortus Botanicus.

At the end, the street and the canal make a turn in a northeasterly direction to the Plantage Middenlaan.

The Dutch journalist, writer and director Milo Anstadt (1920–2011) lived with his family at Plantage Muidergracht 89.

The sculptor Theresia van der Pant (19242013) lived and died at number 153.