P. hyphessobryconis is a known pathogen of the zebra danio, a model organism used in many research laboratories.

[4] Diagnosis of Pleistophora hyphessobryconis infection is made through visual microscopy of damaged tissue.

[5] The Lilly-Twort and Accustain Gram stain procedures are effective at visualizing spores within infected tissue.

[2] Some veterinary diagnostic laboratories offer PCR testing for this parasite.

Fish infected with P. hyphessobryconis should be removed from the enclosure to prevent the spread of the disease.