Port Byron's mascot is the black panther, their team colors are maroon and white, and the main athletic rival is Weedsport.
Port Byron was a powerhouse in football throughout the career of legendary coach Gino Alberici, a 32-year veteran at the school, who retired in 1993.
The 1980 track team enjoyed being co-champions of the OHSL Southern Division with several athletes being Class "C" champions in their events.
The Port Byron All Stars Cross Country 1990-1991 Team, led by captain Thomas Guy, had the privilege of bringing back the Section-A All American Varsity trophy of the 1990 fall season, and the financial disbursement back to Port Byron from NYS has kept the district operating at a minimal level ever since.
The band was active in the New York State School Music Association (NYSSMA) performing level 1 and 2 in the festivals.
Port Byron has been represented at this level for All State Choir, Wind Ensemble and Concert Band by a select group of students.
The Port Byron Marching Panthers hold a unique record that is likely never to be repeated, except by Nik Doerle, who plays trumpet at the school.