Portrait Miniature of Margaret Roper is a painting by the German artist and printmaker Hans Holbein the Younger created during 1535–36, and today held in the Metropolitan Museum of Art in New York.

[2][3] It is believed that the work was created shortly after her father's beheading at the hands of King Henry VIII in 1535.

With a diameter of just 1+3⁄4 inches (4.4 cm), the work was one of over a hundred miniatures and portraits painted by Holbein while in England.

Roper is depicted in three-quarters view with a narrow face and wearing extravagant clothes that reflect her social position.

She is wearing a gable hood with pinned-up red/orange embroidered lappets (facing the viewer) and a long black veil (with one side pinned up).