The Portrait of Sir Richard Southwell is a painting by the German Renaissance master Hans Holbein the Younger, executed around 1536–1537.

It belongs to Holbein's mature career, and a preparatory drawing of the painting (with the inscription "Southwell Knight") exists in the Royal Collections of the Windsor Castle.

The Louvre houses a copy brought to Paris during the Napoleonic invasions of Europe.

Richard Southwell was a privy councillor of Henry VIII of England and was portrayed by Holbein, who had become court painter a short time before, in 1536, as recorded in the inscription.

The latter reads: X° IVLII ANNO H[ENRICI] VIII XXVIII° / ETATIS SUAE ANNO XXXIIIThe painting had once an ebony frame, of which only the silver decorative medallions remain, with the coats of arms of the Medici, Arundel, Southwell and the painter's name.