While not engaging with the Potano, the Spanish incursion spread new infectious diseases and incited warfare by competing tribes in the area.

The army passed through Potano towns that the Spanish called Itaraholata (or Ytara) (probably in western Marion County), Potano (near present-day Evinston),[4] Utinamochana (or Utinama or Untinamocharro) (west of present-day Gainesville, near Moon Lake), Mala-paz (near the present-day city of Alachua) and Cholupa (in the Robinson Sinks near the Santa Fe River in northwestern Alachua County).

To punish them, a second Spanish expedition attacked and killed many Potano and drove the rest from their towns.

After that attack, the town of Potano was moved to the Fox Pond site near the Devil's Millhopper northwest of Gainesville.

A visita (a mission without a resident missionary) named Apula was established in the town of Potano, but was destroyed in the Spanish raid of 1584 or 1585.

After the rebellion, the Spanish re-established the Potano missions (San Francisco and Santa Ana).

Following the Timucuan rebellion, the Spanish made many land grants to their colonists in areas no longer used by the reduced Potano population.

Much of the evidence for these ranches was recorded as Potano complaints to Spanish officials that cattle were running loose and eating village crops.