It is listed as Threatened under the United States Endangered Species Act; there is a major debate about whether it is a valid taxon.

They hibernate an estimated 7–8 months - from September or October through late May or early June[6] - building themselves a soft vegetation nest, typically at a location with a northerly aspect.

[8] Meadow jumping mice like to eat mostly plants, insects, spiders, and slugs.

In June 1998, a preble's meadow jumping mouse was found with a case of Myiasis.

In Colorado, known habitat areas include the Rocky Flats National Wildlife Refuge, South Boulder Creek and the St. Vrain Valley.