[1] The species is found in North America, from Canada to Mexico, nesting deep within the ground.

Unusual among ants, Prenolepis imparis prefers lower temperatures, including near freezing, and is only active outside the nest during winter and early spring.

[2] Prenolepis imparis enters a hibernation-like state called aestivation during the summer.

P. imparis secretes an opaque white liquid from its abdomen as a defense against other ants, including Linepithema humile.

[3] The defensive secretions appear to be derived anatomically from the Dufour's gland and has been shown to contain a mixture of alkanes and alkenes including hexadecene, octadecene, tetradecene, octylcyclohexane, and hexadecane.