As a result, it is employed in cloning and sequencing efforts in plants, fungi, and mammals with minor alterations.
The DNA of interest may be a plasmid insert, a PCR product or a fragment representing a gap when sequencing a genome.
For example, the gene for a disease may be located near a specific marker such as an RFLP on the sequence.
To put it another way, it's utilized to find, isolate, and clone a specific sequence existing near the gene to be mapped.
This method necessitates the discovery of a marker that is firmly related to the mutant locus.
This means you have to break down longer DNA molecules, clone and subsequently sequence them.
[7] The first is called chromosome (or primer) walking and starts with sequencing the first piece.
This technique doesn't require much assembling, but you need a lot of primers and it is relatively slow.
To resolve this problem, a first draft is made and then critical regions are resequenced using other techniques such as primer walking.