Chromosome jumping

[5][6] In order to construct and characterize a library based from NotI-digested human DNA, random clones were analyzed by restriction mapping.

[3] Due to the wide distribution of fragment sizes made by the complete digestion with NotI, the library was constructed into two fractions, low and high plasmid concentration.

[3] Clones that possessed unique end fragments were then analyzed by hybridization to Pulse Field Gradient (PFG) Southern blots.

[3] Examining the results gathered for single and double digests of human DNA with enzymes NotI, BssHII, and NruI, a restriction map with 850 kb was region containing the linking and jumping clones were created.

Chromosome jumping libraries help address the complication of standard cloning techniques with large molecular distances.

[8] Both these complications, traditional cloning techniques are unable to process because large yield of exons would have to be visible to produce a signal for the cystic fibrosis gene to be identified and DNA would have to be free of any repetitive elements.