[1] After the death of her mother in 1551, she as well as her siblings were placed in the care of Christina Gyllenstierna and then under her aunts Brita and Martha Leijonhufvud before her father's remarriage to Catherine Stenbock.

In 1556, she and her sisters were given a dowry of 100.000 daler, had their portraits painted and their personal qualities described in Latin by the court poet Henricus Mollerus, and introduced on the Dynastic European marriage market.

The marriage is described as a happy one, and Anna reportedly acted as the adviser of George John, trying to use her influence to restrain his many adventurous and expensive projects.

[2] One of the most costly expenses was the foundation of the city of Pfalzburg (1570) in Northern Alsace and where their court often resided in the castle La Petite Pierre (Lützelstien).

In 1579, her dowry was evidently wasted, as she and her spouse could not afford to attend the wedding between her brother Charles and Maria of Palatinate-Simmern in accordance with their status.

[2] In order to solve the debt problems, Anna dissolved the court and lived in the household of her brothers-in-law until she could afford to have her own household again: she also entered negotiations with her brother Charles IX and queen dowager Gunilla Bielke in order to be given the Swedish funds which she managed to have John III promise to send her before his death.

Anna was described as a faithful and dutiful, compassionate and cooperative but also firm: she was afforded to recognition of having successfully solved an extremely difficult situation during her regency because of her personal qualities and by emphasizing her status an authority as the central figure and matriarch of the dynasty.