It was formed on 14 May 1608 by Frederick IV, Elector Palatine in order to defend the rights, land and safety of each member.
Firstly, the Holy Roman Emperor Rudolf II and Bavarian Duke Maximilian I reestablished Catholicism in Donauwörth in 1607.
Secondly, by 1608, a majority of the Imperial Diet had decided that the renewal of the 1555 Peace of Augsburg should be conditional upon the restoration of all church land appropriated since 1552.
[1] Members of the Protestant Union included the Palatinate, Neuburg, Württemberg, Baden-Durlach, Ansbach, Bayreuth, Anhalt, Zweibrücken, Oettingen, Hesse-Kassel, Brandenburg, and the free cities of Ulm, Strasbourg, Nuremberg, Rothenburg, Windsheim, Schweinfurt, Weissenburg, Nördlingen, Schwäbisch Hall, Heilbronn, Memmingen, Kempten, Landau, Worms, Speyer, Aalen and Giengen.
It allied some Protestant states in western, central and southern Germany, and fought against the Holy Roman Emperor under the guidance of Sweden and France, which were at the same time parties to that league.