St Edmund Hall, a constituent college of the University of Oxford, is located on the southern end of this street.
At the southeastern end of Queen's Lane is a junction onto the High Street.
To the west is Queen's College and to the east on the corner is the Queen's Lane Coffee House, a historic coffee house dating from 1654, claimed (along with others) to be the oldest in Oxford.
The lane is largely surrounded by high stone walls with few windows, but with some good examples of gargoyles, a feature of Oxford college architecture.
Past the bend at the middle of the current Queen's Lane it was called Torald Street.