The R564 is a Regional Route in the City of Johannesburg Metropolitan Municipality in Gauteng, South Africa.

[1] Its south-western end is a junction with the R41 (Main Reef Road) in Roodepoort, in the southern part of the Florida suburb.

Here, it becomes known as Northumberland Avenue and heads north-east through the suburbs of Sundowner and Northgate to reach a junction with the R512 (Malibongwe Drive).

[1] It continues in an east-north-easterly direction from the R512 junction as Witkoppen Road, through the suburbs of Northriding, Bloubosrand, Johannesburg North, Jukskei Park, and Fourways.

Here, it meets the southern terminus of the R552 (Cedar Road) and crosses the R511 (Winnie Mandela Drive).