The R65 is a provincial route in Mpumalanga, South Africa that connects Ermelo with the Eswatini border at Sandlane, via Amsterdam.

[1] The R65 begins just north-east of the Ermelo town centre, at a junction with the N17 national route.

It heads eastwards from the N17 junction for 73 kilometres to reach a junction with the R33 route in the town of Amsterdam.

The R65 continues east-north-east for 17 kilometres to reach the Nerston Border Post, where it crosses into Eswatini and becomes the MR19 route.

The border on the Eswatini side is named Sandlane.