The film marks a significant shift in Daffy's personality, going from being the insane "screwball" character who (like Bugs) overwhelmed his adversaries, to a much more flawed individual, full of greed and vanity and desire for attention under the spotlight.

Daffy Duck orchestrates a ruse to lure Elmer Fudd to Bugs Bunny's burrow, initiating a cycle of mistaken identity.

Through a series of costume changes and sign manipulations, Bugs and Daffy perpetuate the confusion, ultimately turning the tables on Elmer and subjecting him to their own brand of humor.

[4] The "rabbit season/duck season" argument from this short became one of the references in the Looney Tunes franchise to have been analyzed both by scholars and by Jones himself (although this gag was actually used by Daffy against Porky six years earlier in the cartoon Duck Soup to Nuts).

According to an essay by Darragh O'Donoghue, Rabbit Fire "stands in close relation to human experience, striving and generally failing to grasp an elusive quarry or goal.

The style, setup, and plot of Rabbit Fire were adapted into the opening sequence of Warner Bros.' 2003 film Looney Tunes: Back in Action.